Download the science mobile app for android devices. Data was generated on either the bd accuri c6 plus or the bd accuri c6 as indicated in figure legends. Bd accuri c6 flow cytometer system for sale youtube. Take a leap in flow cytometry with the bd accuri c6 plus. Jan 11, 2020 buy used bd accuri c6 at great prices from certified genetool. Follow the instructions in the bd cba kit manual for preparation of samples and use of reagents. The bd accuri c6 flow cytometer is easy to use and allows student access to the science of flow cytometry.
Could someone share herhis experience with basic facs. Bd accuri c6 system 32bit software is not forward compatible with bd accuri c6 plus 64bit software and the fcs 3. Catalog number bd accuri c6 plus this product is no longer available on biocompare. Brand bd contents for use with bd accuri c6 plus, bd facslyric ruofor us only, and bd facsmelody. All reagents and kits are compatible with both bd accuri c6 plus and accuri c6 flow cytometer systems. Lightweight, with a small footprint and straightforward maintenance, its often the cytometer of choice. It also has two lasers, 6 parameters which fit your need.
The bd accuri c6 plus only weighs only 30 pounds and has a compact footprint of 11 x 14. Evaluation of an easy and affordable flow cytometer for. Enhanced sensitivity, reliability, and capabilities bring flow. Bd accuri c6 flow cytometer from bd biosciences selectscience. New plex 11dbb56bd863e6bb created by fcap array infinite 119. Turn on the computer and log in with your ucf info. Bd accuri c6 plus software features an intuitive user interface.
Breakthrough with the bd accuri c6 plus cell analyzer. Bd accuri c6 software templates matched to each kit include predefined workspaces, markers, regions, gates, and parameter names for quick and easy setup and analysis. Bd accuri c6 plus flow cytometer with its compact 11 x 14. Guide for using bd cytometric bead array cba kits with. First, fixedvoltage detectors simplify data collection and reduce the potential for data loss due to signal over or underamplification. From basic to more challenging applications, the bd accuri c6 plus personal flow cytometer is a powerful benchtop tool that provides you with the versatility and the analytical power of a cytometer twice its size. Bd biosciences accuri c6 personal flow cytometer overview. The intuitive interface of the software guides the user through workflows.
Valve issues, fluidics leaking onto electronics, flow cell. Cytometry bd accuri question hi all, as always, the list has been. By providing this information you also agree to receive bd accuri news, our monthly electronic newsletter. The bd accuri c6 plus is an ideal benchtop platform for core immunophenotyping studies and is configured for rapid and accurate analysis of up to six parameters at the singlecell level. The bd 6 peak and 8 peak beads will be used to verify it. Guide for using bd cytometric bead array cba kits with the bd accuri c6 flow cytometer subject. Any one have good or bad experiences with the new bd accuri c6 plus. A broad dynamic range of detection makes it easy to analyze cells as varied in size as platelets and eosinophils in the same data file. The bd accuri c6 is a personal flow cytometer that brings flow cytometry within reach by being easy to use, simple to maintain, and affordable. Samples were collected on the bd accuri c6 flow cytometer using the kit template and analyzed using bd accuri c6 software.
Dec 30, 2016 bd accuri c6 flow cytometer system for sale at the lab world group. Bd accuri c6 training and applications support at customer sites will be offered based on the availability of staff with product knowledge. Bd accuri c6 flow cytometer certified with warranty. The bd accuri c6 has the sensitivity, reliability, and. This portable and compact system holds the analytical power of a cytometer twice its size. Free bd accuri c6 software templates simplify data collection and analysis. It also includes common troubleshooting help and service information. On the bd accuri c6, the default setting of a primary threshold on fsch of 80,000 indicates that the system will record an event only if it has an fsc value of 80,000, regardless of any other parameter values. The bd accuri c6 software installer is on the cd that came with the c6. Ive recently started doing flow cytometry bd accuri c6 in a core facility at my. Bd biosciences offers a range of costeffective immunology and stem cell kits ideally suited for the bd accuri c6 that include needed reagents and buffers to assess multiple cell surface and intracellular phenotyping markers. Be sure to perform all requisite system startup and cleaning functions. As a result, microsoft will no longer provide security updates or support for devices running windows 7.
December 2017 bd accuri c6 flow cytometer repair mafiadoc. Bd accuri c6 system 32bit software is not forward compatible with bd. Remove and discard the black sip cover and place a tube containing at least 2ml of filtered, deionized water on the sip. The bd accuri c6 will be recertified to oem specifications by our inhouse service technician. When calling the technical support of bd, i was told that old generation can only run on 32bit. The accuri c6 has small foot print and is able to count wide range size of particles in volume methods dont need add beads. Several features of the bd accuri c6 flow cytometer make it an ideal platform for differential analysis of human peripheral blood samples. Add 35l of firefly particles to the well, and apply vacuum to remove all liquid from the well. Cited in over 9,000 publications, the bd accuri c6 plus enables you to go beyond your current research potential. Bd biosciences bd accuri c6 plus community, manuals and.
Enhanced sensitivity, reliability, and capabilities bring flow cytometry even more within reach for new and experienced flow cytometry researchers. Connect the cable to the socket at the back of the bd accuri c6 and switch on the bd accuri c6. Warning failure to keep the mat clear of plates, tubes or any other. Bd accuri c6 flow cytometer instrument manual bd accuri c6 software will trigger the notification based on the volume of sheath run through the system or on the date since maintenance was last performed.
It may be suitable for those labs that require more routine applications, andor dont require a large number of fluorescence. Apr 07, 2020 bd accuri c6, bd facsaria ii, bd facscalibur, bd facsjazz, bd facs sample prep assistant ii and bd facsverse are discontinued. To recreate bd accuri c6 software plots in flowjo with the same axes scaling as the original bd accuri c6 software file, note the channel range used to view each parameter figure 1. Bd accuri c6, bd facsaria ii, bd facscalibur, bd facsjazz, bd facs sample prep assistant ii and bd facsverse are discontinued. An example bd accuri c6 software file of a fourcolor analysis of human peripheral blood hpb 4 color example. This protocol also assumes a completed assay transferred into facs or eppendorf tubes, with particles suspended in run buffer. The bd csampler will auto matically align itself and then remain in the home position figure 8 9. The subsystem validation of solidstate detector in bd. Bd accuri c6 manual certainly provide much more likely to be effective through with hard work. The bd accuri c6 flow cytometer along with the bd cytometric bead array reagent sets, provides researchers a.
The bd accuri c6 is a personal flow cytometer that puts flow. Bd accuri c6 plus flow cytometer from bd biosciences. Bd accuri c6 software will display this notification based on whichever trigger is reached first. Protocols, tools, documentation, and other information to support cell analysis using the bd accuri c6 plus research cell analyzer. Please complete the following information to request a demonstration of the bd accuri c6 plus flow cytometer. All reagents and kits are compatible with both bd accuri c6 plus and accuri c6 flow. December 2017 bd accuri c6 flow cytometer repair brad elmore before working on instrument. Please contact your regional customertraining center for more information. Buy used bd accuri c6 at great prices from certified genetool. Optimize your flow cytometry best practices for sample preparation, staining and analysis duration. Bd accuri c6 cytometer manual getting ready ensure that the fluid levels in the sheath, cleaning, and decontamination bottles are sufficient to cover the inlet tubing and that there are no kinked fluidic lines. The c6 uses two lasers, red and blue, to record events and has 4 fluorescence channels for a wide range of fluorescence detection. Bd accuri c6 with single tube loader protocol this protocol contains instructions for setting up an accuri c6 with tube loader. Software options and instrument controls are clearly visible from the softwares tabbed interface.
Our neuroimmunology lab currently has a bd accuri c6 that has broken about every year and a half since its purchase about 5 yrs old. Microsoft will end support for the windows 7 operating system os after january 14, 2020 and for windows embedded standard 7 service pack 1 on october, 2020. Nov 09, 2017 optimize your flow cytometry best practices for sample preparation, staining and analysis duration. System has been fully validated and comes complete with warranty. We further ran the swap test and repeatability as well as ran test at the operational temperature range of cytometer. The bd accuri c6 plus personal flow cytometer is the newest generation of the bd accuri platform. This protocol also assumes a completed assay in a 96well plate, with particles suspended in run buffer. Platforms referred to as bd accuri represent both the bd accuri c6 plus and bd accuri c6. Can anyone suggest good free flow cytometry software that can read. It is a small but powerful accuri c6 comes computer loaded with bd accuri c6 plus software. Setupverification protocol on your bd accuri c6 cytometer using particles. The purpose of this guide is to illustrate the setup procedure on the bd accuri c6 flow cytometer.
Using the bd accuri c6 cytometer for rapid and accurate analysis of the nuclear dna contents of flowering plants download accuri white paper on rapid analysis for. After contracts terminate, bd will support the bd accuri c6 system for as long as reasonably possible, depending on parts availability and other matters. Many bd accuri c6 customers can begin collecting and analyzing data with the help of a quick start guide. Even new flow cytometry users find it so easy to learn and use that. Full proteomics results are available for download as a scaffold. Second, the instruments broad dynamic range makes it easy to analyze populations as varied in size as platelets and. The bd accuri c6 has the sensitivity, reliability, and capabilities needed to bring flow cytometry to the next level. All reagents and kits are compatible with both the bd accuri c6 plus and bd accuri c6 flow cytometer systems. The bd accuri c6 flow cytometer along with the bd cytometric bead array reagent sets, provides researchers a powerful flow cytometry toolset for cell analysis. Start up the accuri system and open the cflow software.
The acquisition and analysis of data were compared for the flow cytometers. The bd accuri cflow importer reads the fcs data directly from the bd accuri cflow file, so you do not have to export individual files. Bd accuri c6 flow cytometer system for sale at the lab world group. Even new flow cytometry users find it so easy to learn and use that most can collect and analyze data in less than an hour. Biocompare is the leading resource for uptodate product information, product. The bd accuri c6 flow cytometer reports arithmetic means. The following conditions may indicate a problem with the cytometer or the validation beads. If you have any questions regarding this matter, please see the following bd accuri frequently asked questions or contact bd service contracts administration 1. And you should get the bd accuri c6 manual driving under the download link we provide. Bd bioscience and pavilion integration corporation pic use bd instruments accuri c6 plus as test tools to test the ssd of pic vs.
For information on using bd accuri c6 software, see the bd accuri c6 software. As a result, most novice flow cytometry users find it so easy to use, that they can collect and analyze data in less than an hour. To install bd accuri c6 software, run bdaccuric6softwareinstaller. At bd, we seek to usher in a new era of healthcare by bringing medical products, capabilities and solutions to every corner of the world. Bd accuri c6 software has an intuitive user interface that was developed based on hundreds of hours observing researchers using flow cytometers. Examples of axes ranges used in a bd accuri c6 software file. Lab closures caused by covid19 longterm shutdown quick. Bd pharmingen, fitc brdu flow kit, 559619 download. Download pdf copy download brochure the bd accuri c6 is a personal flow cytometer that brings flow cytometry within reach by being easy to use, simple to maintain, and affordable. Download the catalog of available kits and templates, or select from the following table.
For everyone, whether you are going to start to join with others to consult a book, this bd accuri c6 manual is very advisable. Storage buffer phosphate buffered saline pbs with bovine serum albumin bsa and 0. Since this is a special order c6 we have on site support from a field service engineer. Bd accuri c6 software will display this notification based on. A new lab toy to make it big in the last 510 years is the accuri c6 cytometer now under the bd umbrella, a lowcost instrument in comparison to the big boys. At the time bd facs sample prep assistant ii was placed on the union market, the instrument was ce marked in compliance with the european in vitro diagnostic medical device directive 9879ec. Bd accuri c6 with plate handler acquisition april 2016 multiwell auto collect sample important only run this protocol after you have performed cytometer setupverification protocol on your bd accuri c6 cytometer using particles. In this example, the file is fcs express import demo. Lin fsch, channels 0 to 5 x 107 lin ssch, channels 0 to 2x 107. Bd accuri c6 plus software files can be exported in fcs 3. Threshold and analysis of small particles on the bd accuri.
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